PCR Hybrispot Zoonoses (HybZOO)
Tick extraction + mutiplex PCRs + dotblot for 7 pathogens: Erhlichia, Anaplasma, Bartonella, Borrelia burgdorferi sl, Rickettsias (Richettsia, Spotted fever group & Typhus group), Coxiella, Francisella
HybZOO: Request Form from EU
HybZOO: Request Form from USA
Sample required: blood, saliva, biopsy, swab, as per need Blood: 1 EDTA. Tube. Stable for 48hours. For longer periods freeze at –20°C or –80°C. Saliva , biopsy, swab: sample in a sterile, dry tube (no buffer or fixative of any kind). Stable for 24 hours. For longer periods freeze at -20°C or 80°C
Treponema IgG & IgM mmunoblot (SeroTRPN)
SeroTRPN: Request Form from EU
SeroTRPN: Request Form from USA
SERUM-BASED TESTS : Sample required: 2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For longer periods, centrifuge, aliquot & freeze. IMMUNOBLOTS (WESTERN BLOTS) WITH DIAGRAM
COMBO TORCH IgG & IgM immunoblot (SeroTORCH)
Toxoplasma gondii, rubella virus, cytomegalovirus (CMV), herpes simplex virus type 1 & 2 (HSV-1/2)
SeroTORCH: Request Form from EU
SeroTORCH: Request Form from USA
SERUM-BASED TESTS : Sample required: 2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For longer periods, centrifuge, aliquot & freeze. IMMUNOBLOTS (WESTERN BLOTS) WITH DIAGRAM
Mycobacterium QuantiFeron GOLD test (MycoB)
Mycobacterium QuantiFeron GOLD test: detects responses in vitro to mycobacterium infection by measuring IFN-γ response to either ESAT-6 or CFP-10 antigens
MycoB: Request Form from EU
MycoB: Request Form from USA
Special tubes required; shipped upon request; tubes must be stored for 16-24h at 37°C after the blood draw .
Borrelia IgG & IgM immunoblot (SeroBORREL)
Borrelia IgG & IgM immunoblot (SeroBORREL) Borrelia burgdorferi ss, B. afzelii, B. gariniii, B. spielmanii, B.bavariensi
SeroBorrel: Request Form from EU
SeroBorrel: Request Form from USA
SERUM-BASED TESTS : Sample required: 2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For longer periods, centrifuge, aliquot & freeze. IMMUNOBLOTS (WESTERN BLOTS) WITH DIAGRAM
Chlamydias IgG & IgA immunoblot (SeroCHLAM)
Chlamydias IgG & IgA immunoblot (SeroCHLAM) Chlamydia pneumoniae, Chlamydia trachomatis, Chlamydia psittaci
SeroCHLAM: Request Form from EU
SeroCHLAM: Request Form from USA
SERUM-BASED TESTS : Sample required: 2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For longer periods, centrifuge, aliquot & freeze. IMMUNOBLOTS (WESTERN BLOTS) WITH DIAGRAM